Building a model: developing genomic resources for common milkweed (Asclepias syriaca) with low coverage genome sequencing

Table 1 Primers used for Sanger sequence finishing of the Asclepias syriaca chloroplast genome

Region spanned	Orientation	Primer Sequence (5'-3')	Annealing Temp. (°C)
ndhC-trnV	FORWARD	GCTCGATTCGAATTGTCAAGTCATCC	57
	REVERSE	AGGCCCTCCGATAGGATACTCAAA
trnQ-psbK	FORWARD	AAACCCGTTGCCTTACCACTTG	57
	REVERSE	CACGGGTATGACTGGCATAACATC
rbcL-accD	FORWARD	ATCCGTGAGGCTAGCAAATGGAGT	59
	REVERSE	AGGCCCTAGTCCACCAATATGGAA
accD	FORWARD	TAATAAACAATGCTAGCGATTGGG	57, 61
	REVERSE	TACAATTAGATCTTAGGTCGCCAA
	FORWARD	GAACCTACACGCAAGCTAGTG
	FORWARD	TAAATGGCATTCCCATAGCAGT
	FORWARD	TGATATCGATGCGCAAGCTAG
	REVERSE	CCTATAAATGTAACTAGCACTGCA
	REVERSE	GGTAGCGTATTCAATCAAACGG
	REVERSE	CCAAGTATCCGGATCGATCGA
clpP	FORWARD	AATTGTATACATAATGGGCTGGT	57
	REVERSE	TATTTATTCATTGCCCTGTTCGT
	FORWARD	AACTACTATGATGGCTCCGTTG
	FORWARD	CATAAGACCCATAATTTGATTTGAG
	FORWARD	GTTTATGCTGTACTCCGGGTA
	REVERSE	GGTCAATTTTATTGTAAAGCCGTAT
	REVERSE	TATGCAATTTATAAAACCAGATGTCC
	REVERSE	GTGAATTGAAAAAGTAGAACGTCAT
rps8 -rpl14	FORWARD	CTCAGCAATAGTGTCTCTGCCCAT	57
	REVERSE	AGAGCTGTAATTGTGCGTACCCGT
ycf1-ndhF	FORWARD	TCCATCCATATCCCAATTCCATTCA	55
	REVERSE	GGGCATTAGAGGATTGGCTAAAGT
	FORWARD	ACGCCTCTGCATCTAGTATTG
	REVERSE	GGATCTTTTGTCGAGAGCTTC
ndhG-ndhI	FORWARD	ATGCGGGCGGGTTGATATATGGTT	59
	REVERSE	ATTGCTTTGGGTCGGTTACCAACGTC
rps15-ycf1	FORWARD	GGCCTAATTGATACCTCAGCGGTT	58
	REVERSE	TTATCAACGATGGAACCGCCCACT
	FORWARD	TATGTCTCGGTCTAGGAGAGG
	REVERSE	TGTACAAGTTGATTCAGGCGA

For each region, additional primers appearing after the two original PCR primers are internal sequencing primers.

ISSN: 1471-2164