Figure 4

PCR confirmation of the 89 K polymorphisms in the SS2 strains. (A) Schematic representation of SS2 genomes with 89 K and relevant primers (shown as black triangles). (B) PCR detection of 89 K with primers P1&P17. (C) PCR detection of 89 K in strains 606 and 1941. (D) PCR detection of 89 K in 8011. (E) PCR detection of 89 K in T15. The primer combinations used in the PCR analysis are marked on the lanes. The asterisk represents strain 05ZYH33 being used as the template. M, the DNA marker, from (top to bottom) 4.5, 3, 2, 1.2, 0.8, 0.5, and 0.2 kb, respectively.