Figure 4

Fluorescent in situ hybridization (FISH) analysis of four candidate genes that are up-regulated in melanoma and have a high M-measure. Two probes were used for each gene: one probe covers the 3'-end and the other probe covers the 5'-end. For each gene in each sample, 200 cells were analyzed, and the frequency of aberrations is reported. No translocation events were detected. The spectrum of aberrations of the selected four genes is highly heterogeneous, such that each cell has a different number of amplifications (black and grey bars). With the exception of YUDOSO, at least one locus among FAM84B, ETV4 and EZH2 are aberrant in more than 75% of the cells in the tumor samples.