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Table 5 Sites experimentally tested for in vivo AtoC binding

From: Escherichia coli genome-wide promoter analysis: Identification of additional AtoC binding target elements

Result

Site

Gene

Motif

Strand

Site distance from TSS (Refseq)

Gene product

+

GCTATGCAGAAATTTGCACA

atoD

1,2,3,4

(+)

-184

-165

acetyl-CoA: acetoacetyl-CoA transferase, subunit

+

GCTATGCAGAAAATTGCGCA

atoD

1,2,4

(-)

-163

-144

acetyl-CoA: acetoacetyl-CoA transferase, subunit

+

GCTATAGAAATAATTACACA

dmsA

1,2

(-)

-69

-50

dimethyl sulfoxide reductase, anaerobic, subunit A [25]

+

GCCATGCGGGGATTTAATCA

puuP

1

(+)

-148

-129

putrescine importer [24]

+

GCCGTCCAGATGTTTACACA*

metR-metE

1

(-)

-87, -170

-68, -151

transcriptional activator [30]

+

GCGATTGTAGGGATTGCTCA*

trmA-btuB

1,(2)

(+)

-49, -340

-30, -321

tRNA (uracil-5-)-methyltransferase/ outer membrane transporter [31]

+

ACTATACGGAAAATTCCACT*

ykgA-ykgQ

1,2

(-)

-235, -55

-216, -36

Putative transcriptional activator [32]

+

GTGATGGGATTATTTGATCT*

aegA-narQ

1

(-)

-147, -79

-128, -60

predicted fused oxidoreductase: FeS binding subunit and sensory HK in TCS with NarP [33, 34]

+

GCCTCGGAGGTATTTAAACA*

cpxR-cpxP

1

(-)

-21, -149

-2, -130

response regulator in TCS with CpxA/ periplasmic protein; combats stress [36]

+

GCGAGGCGGGTAATTAGACA

ymiA

1

(-)

-199

-180

small predicted membrane protein [35]

+

GGCCCAATTTACTGCTTAGG

crr

1,2

(+)

-28

-9

glucose-specific PTS component [26]

+

GCGGTGCAGGAGATTGCACA

fliT

1

(+)

127

146

predicted chaperone (flagellum) [46]

+

CCGGTCCAGGAATTTACTCA

narG

1

(-)

2

21

nitrate reductase 1, alpha subunit [23]

+

GCGAAACGATTAATTACACA

ycjM

1

(+)

94

113

glucosyltransferase predicted [64]

+

ACTGTACAGAAAGTTGCTCA

yeaM

1

(-)

668

687

transcription regulator (predicted) [65]

+

GCGGGTCAGGTAATTGCACA

gadA

1

(-)

959

978

glutamate decarboxylase A, PLP-dependent [66]

+

GACATGCAGTTGATTACACA

eutR

1

(+)

783

802

predicted transcription regulator [67]

+

GCGATCCAAAAGTTTACTCA

narZ

1

(+)

0

+ 19

nitrate reductase 2 alpha subunit [23]

+

CGGAGATTTCCCGCAAAGCC*

rtcB-rtcR

2

(+)

-145, -64

-126, -45

sigma-54-dependent transcriptional regulator of rtcBA [27]

+

CGGCAAGTTTCGACATTGCC*

putA-putP

2

(+)

-398, -45

-379, -26

fused transcriptional regulator/proline dehydrogenase/ carboxproline:sodium symporter [37]

-

GGGCATTTTCCTGCAAAACC*

rhaT-sodA

3

(+)

-167, -138

-148, -119

L-rhamnose:proton symporter/superoxide dismutase [39, 40]

-

GGATGATGTTCTGCATAGCA

adhP

3

(+)

-35

-16

ethanol-active dehydrogenase/acetaldehyde-active reductase [38]

-

TTTATAGAAATAGATGCACG

nirB

4

(-)

-205

-186

nitrite reductase, large subunit, NAD(P)H-binding [41]

+

TATGTGTAGAAAATTAAACA

borD

4,3

(+)

-59

-40

DLP12 prophage; predicted lipoprotein [28]

+

GTTCAGTATAAAAGGGCATG

acrD

2

(+)

-128

-109

aminoglycoside/multidrug efflux system [29]

-

GAAATAGAAATAGTTGAAAG

ykgE

4

(-)

-472

-453

predicted oxidoreductase [32]

+

CCGGATAAGGCGTTTACGCC

yjcH

4

(+),(-)

-114, -122

-95, -103

conserved inner membrane protein/ acetate transport [44]

-

CCGGATAAGACGTTTACGCC

yafJ

4

(+)

-193

-174

Unknown function, similarity to type II amidotransfease

+

CCGGATAAGGCGTTTACGCC

sseB

4

(+)

-125

-106

rhodanese-like enzyme [68]

  1. Motif hits experimentally tested by ChIP analysis for in vivo AtoC binding. All annotations were provided by the Refseq database (Escherichia coli K12, Refseq id: NC_000913). Underlined: hits in gene-encoding regions. *These hits represent a unique site per pair, which could be attributed as cis-regulatory element to both flanking genes. As predicted by the GO Term analysis many of the targets are transcription factors, transporters and enzymes involved in oxidoreduction.