Figure 4

The repertoire of GPCRs expressed by mature cerebellar granule neurons is mostly determinate after 3 days in vitro (DIV 3). To monitor global changes in GPCR expression, GPCRs were ranked by their expression levels, and the absolute difference in the ranks of each GPCR between 2 consecutive time points, i.e. DIV 0-1, DIV 1-2, DIV 2-3 and so on, were recorded. Box plots were drawn to visualize the distributions of the differences in GPCR expression levels between 2 consecutive DIV. Box plots are shown for both the whole GPCR set (Figure 4A) and for the 50 most abundantly expressed GPCRs at DIV 9 (Figure 4B). Whereas the distributions are comparable for the whole GPCR set, the box plots for the 50 most expressed GPCRs display a decrease in the average difference between 2 consecutive DIV before and after DIV 3, indicating that the repertoire of GPCRs expressed by mature cerebellar granule neurons is mostly determinate at DIV 3, when neurons are not morphologically mature yet.