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Table 1 Comparative analysis of the accuracy and quality of sequences

From: Accuracy and quality assessment of 454 GS-FLX Titanium pyrosequencing

  # of sequences % of error-free sequences # of positions Insertions Deletions Mismatch Ambiguous Total % of error
GS20 (101) 34015 82.00% 32801429 0.18% 0.13% 0.08% 0.10% 0.49%
Ref 1 (101) 16052 87.12% 1605640 0.15% 0.05% 0.01% 0.01% 0.22%
Ref 2 (101) 16466 60.01% 1600327 0.42% 0.23% 0.04% 0.01% 0.70%
Ref 3 (101) 12215 72.96% 1228804 0.17% 0.19% 0.01% 0.01% 0.38%
Ref 4 (101) 9908 56.43% 984452 0.30% 0.37% 0.03% 0.00% 0.70%
Ref 5 (101) 15880 50.93% 1595718 0.34% 0.48% 0.05% 0.01% 0.88%
Ref 6 (101) 15716 75.17% 1581075 0.25% 0.10% 0.00% 0.01% 0.36%
Total 86237 67.57% 8596016 0.27% 0.23% 0.02% 0.01% 0.53%
Ref 1 (572) 16052 6.75% 5359696 0.52% 0.46% 0.10% 0.12% 1.20%
Ref 2 (552) 16466 9.75% 4789285 0.89% 0.28% 0.10% 0.08% 1.35%
Ref 3 (500) 12215 18.75% 4180478 0.30% 0.35% 0.07% 0.12% 0.84%
Ref 4 (532) 9908 6.88% 2572843 0.56% 0.71% 0.19% 0.11% 1.57%
Ref 5 (592) 15880 7.46% 6171098 0.38% 0.38% 0.06% 0.07% 0.89%
Ref 6 (516) 15716 11.81% 6027338 0.60% 0.17% 0.07% 0.04% 0.88%
Total 86237 10.09% 29100738 0.54% 0.36% 0.09% 0.09% 1.07%
  1. The different types of error are detailed for each reference sequence for 454 sequencing. Errors are classified according to the nomenclature used by Huse et al. (2007): insertions, deletions, mismatches and ambiguous base calls (see materials and methods). Error rates are given for two length categories (first 101 bases vs. full length).