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Table 3 Genes, detected both as xenologs and as core genes

From: Blueprint for a minimal photoautotrophic cell: conserved and variable genes in Synechococcus elongatus PCC 7942

Gene name

Protein product

Core genes*

Xenologs¥

Comment

  

1)

2)

3)

4)

A)

B)

C)

 

Synpcc7942_0056

perosamine synthetase

  

yes

29

-2

 

yes

Next to a genomic island, also detected by SIGI-HMM method, true prediction of xenology.

Synpcc7942_0587

valyl-tRNA synthetase

yes

yes

yes

35

-1

 

yes

No further phylogenetic evidence of xenology, false positive prediction of xenology.

Synpcc7942_1007

fumarate hydratase

 

yes

yes

35

-1

N-J

yes

The phylogenetic evidence suggests a true prediction of xenology.

Synpcc7942_1008

formyltetrahydrofolate deformylase

  

yes

33

-1

  

No phylogenetic evidence of xenology, false positive.

Synpcc7942_1064

type I restriction-modification

 

yes

  

1

Blast

yes

The phylogenetic evidence suggests a true prediction of xenology.

Synpcc7942_1080

K+ transporter Trk

yes

yes

yes

35

-2

 

yes

No further phylogenetic evidence of xenology, false positive prediction.

Synpcc7942_1081

hypothetical protein

yes

yes

yes

35

-1

  

No further phylogenetic evidence of xenology, false positive prediction.

Synpcc7942_1132

hypothetical protein

  

yes

4

-2

 

yes

No phylogenetic evidence of xenology, false positive prediction.

Synpcc7942_1660

potassium channel protein

 

yes

 

23

-1

Blast

 

No phylogenetic evidence of xenology, false positive prediction.

Synpcc7942_1851

ferredoxin--nitrite reductase

   

27

-2

  

Maby transferred among Cyanobacteria.

Synpcc7942_1852

precorrin-8X methylmutase

 

yes

yes

35

-1

  

Likely transferred among Cyanobacteria.

Synpcc7942_1853

cobalt-factor II C20-methyltransferase

 

yes

yes

35

-1

  

Likely related to alpha-proteobacteria.

Synpcc7942_1854

precorrin-3 methyltransferase

 

yes

 

26

-2

N-J

 

Closely related to alpha proteobacteria.

Synpcc7942_1855

cobyrinic acid a,c-diamide synthase

 

yes

yes

34

-2

  

Likely transferred among cyanobacteria.

Synpcc7942_1873

two component transcriptional regulator

   

35

-2

  

Likely transferred among cyanobacteria.

Synpcc7942_1887

mannosyltransferase

 

yes

 

8

-1

N-J

 

Likely transferred among cyanobacteria.

Synpcc7942_2188

isochorismate synthase

yes

yes

yes

33

1

N-J

 

Further phylogenetic analyses does not support evidence of xenology, false positive.

Synpcc7942_2436

peptide methionine sulfoxide reductase

 

yes

 

22

-1

N-J

 

Further phylogenetic evidence suggests a true prediction of xenology.

  1. * Criteria to detect coregenes. For each gene, it is shown if it is conserved in 1) chromatophore of P. chromathophora; 2) P. marinus SS 120; 3) CyOGs database; and 4) number of cyanobacterial genomes with homologous genes. ¥Criteria to detect xenologs. A) G+C content in number of SD from the mean; B) unusual phylogenetic relationship by BLAST or Neighbor-joining; and C) Putative Alien (PA) genes.