Figure 2

Results of the fast forward genetics approach. A) First step of the fast forward genetics approach. Single linkage to genomic region was identified for both mutants. The region with low frequency non-reference alleles in the mutant population indicates a common genomic fragment to the mutant pool. Verification in the wild type pool (blue graph) supports this region by showing an overrepresentation of non-reference alleles to balance the Hardy-Weinberg equilibrium. B) Second step of the fast forward genetics approach. Non-reference alleles detected in the genomic enrichment sequencing data are plotted by their location and frequency for mutants (black: non-coding, red: coding, blue: UTR). The black boxes indicate regions where no capture probes could be designed due to repeats and/or other non-unique sequences