Selenium toxicity but not deficient or super-nutritional selenium status vastly alters the transcriptome in rodents

Table 1 Transcripts up-regulated by Se deficiency

Genbank Accession	Gene Symbol	Gene Title	Fold Change¹	adj P-value²	Nrf2 Target³
Mouse Liver
NM_173047	Cbr3	carbonyl reductase 3	7.46	1.7E-05	X
NM_001033336	Abcc4	ATP-binding cassette, sub-family C (CFTR/MRP), member 4	3.73	0.023	X
NM_013560	Hspb1	heat shock protein 1	1.91	0.04
			- - - - - - - - - - - - - - - - - - - - - - - - -
NM_008182	Gsta2	glutathione S-transferase, alpha 2	1.93	0.057
NM_007620	Cbr1	carbonyl reductase 1	1.89	0.057	X
NM_008706	Nqo1	NADPH dehydrogenase, quinone 1	1.85	0.073	X
NM_010357	Gsta4	glutathione S-transferase, alpha 4	1.74	0.17	X
Mouse Kidney
			- - - - - - - - - - - - - - - - - - - - - - - - -
NM_001077353	Gsta3	glutathione S-transferase, alpha 3	1.74	0.076	X
NM_001033336	Abcc4	ATP-binding cassette, sub-family C (CFTR/MRP), member 4	1.89	0.11	X
NM_008706	Nqo1	NADPH dehydrogenase, quinone 1	1.64	0.29	X
Rat Liver ⁴
NM_173323	Ugt2b7	UDP glucuronosyltransferase 2 family, polypeptide B7	2.14	0.01
NM_001009920	Gsta5	glutathione S-transferase Yc2 subunit	3.03	0.02
			- - - - - - - - - - - - - - - - - - - - - - - - -
NM_080581	Abcc3	ATP-binding cassette, sub-family C (CFTR/MRP), member 3	4.29	0.07	X
NM_033443	Arsb	arylsulfatase B	1.40	0.07
NM_017000	Nqo1	NADPH dehydrogenase, quinone 1	1.99	0.16	X

Transcripts that were significantly (above dashed line, P < 0.05) or nearly significantly (below dashed line, 0.05 < P < 0.2) up-regulated in Se-deficient rodent tissues compared to Se-adequate (0.2 or 0.24 μg Se/g diet, for mouse and rat studies respectively). ¹Fold changes determined by analysis of RMA expression data with the Limma package in R software, ²P-values were adjusted for multiple testing, ³Genes known to be Nrf2 targets, determined as described in the text, ⁴ Genes up-regulated in Se-deficient liver from rats in study 2.

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