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Table 2 qRT-PCR validation of 10 differentially expressed genes according to microarray experiment

From: Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

Oligo IDa TIGR IDb Annotation Haic Mdmicroarray M qRT-PCRe
MT014704 TC80412 Peroxidase 48 1.56 2.99
MT006316 TC82368 Disease resistance response protein 39 precursor 16 1.29 0.91
MT014356 TC85808 12-oxophytodienoate reductase (OPR2) 16 1.27 -1.62
MT014726 TC79559 glutathione S-transferase 16 1.96 2.25
MT007682 TC77455 nine-cis-epoxycarotenoid dioxygenase1 16 1.12 0.21
MT000707 TC86358 6a-hydroxymaackiain methyltransferase 16 -2.13 -0.03
MT000671 TC86307 ferredoxin--NADP+ reductase 48 -1.2 -2.3
MT014197 TC85300 chlorophyll a/b-binding protein 48 -1.34 -1.33
MT007613 TC86304 GA protein 48 -2.28 -2.65
MT014137 BF633423 ribulose 1 5-bisphosphate carboxylase small subunit 48 -0.92 0.32
  1. a Oligo ID, identifier of M. truncatula 70-mer oligonucleotides
  2. b TIGR ID, identifier in the TIGR M. truncatula Gene Index;
  3. c Hai = hours after inoculation with M. pinodes
  4. d M = log2 expression ratio P665/Messire
  5. e Primers used to amplify the genes are shown in Table 4