Skip to main content
Figure 4 | BMC Genomics

Figure 4

From: Gene expression profiling of rat spermatogonia and Sertoli cells reveals signaling pathways from stem cells to niche and testicular cancer cells to surrounding stroma

Figure 4

Identification of SSC genes and markers in pre-pubertal type A spermatogonia. (A) Rat Affymetrix probe set IDs for ES, PGC, gonocyte and SSC markers were selected and analyzed for expression in the Spga cell fractions at 9 and 22 dpp. Genes up-regulated or down-regulated at least 2 fold in type A Spga (G9) compared to mitotic germ cells at 22 dpp (G22) are shown in the first two columns. Genes with a detectable or undetectable expression in the two last columns (defined here as the number of Affymetrix Present/Absent in the replicates after MAS 5.0 analysis). (B) The 255 rat SSC-specific transcripts identified in a microarray analysis by Hamra et al. [23] were compared to each respective gene cluster defined in Figure 2B. Left: Shown are the number of transcripts in each cluster (e.g. G9 vs G22) which overlapped with the transcript list by Hamra et al. [23] as compared to the number of randomly expected transcripts (64/17; i.e. 64 observed versus 17 expected in a random distribution). The gradual color code bar shows over-representation (red) and under-representation (blue) of SSC specific gene transcripts described by Hamra et al. [23] in the various clusters defined in this study (Figure 2B). Significance of overlap (p Values) was calculated using a Gaussian hypergeometric test (Fischer exact probability test). Right: Gene list and functional classification of the genes up-regulated in mitotic Spga type A at 9 dpp overlapping with the rat SSC cluster predicted by Hamra et al [23].

Back to article page