Table 2 Oligos and genomic targets used in this study
Primer name | Forward (5'-3') | Reverse (5'-3') | gene target | gene target accession | Phenotype target |
|---|---|---|---|---|---|
AB_UNI | CTTTCAGGKAGTATTGGTCT | CTCATTAGAGTTCACCGAAG | hypothethical protein | ACJ58112 | all |
AB_HYP | CGTCGGTCGGATCCGTGTAT | AAGTAAAGTGGCAGGCGCTT | hypothetical protein | ACJ57861 | invasion |
AB_INV | ATCCATTAGACCTCCCAATC | CATTGTTGATGTGAACTGGA | hypothetical protein | ACJ42779 | invasion |
AB_HEM | TCTCATCTAAAGCACCACCC | TAACCAGTGCTGGTGACATC | hemolysin | ZP_04662166 | colonization |
AB_COL | TCCAATGCCAAATCTTTACT | TGTGTTGAATAACCCTCAAA | alkyl sulfatase | ZP_04663686 | colonization |
AB_ALK | TTCCTGTTATAAGATTGGGAGAA | TTCTGATCCGAGTGATTTATTG | alkyl sulfatase | ZP_04663686 | colonization |
AB_INT | TATCCTTCAGCTTACCCTTCTT | TTTCGACATGTGGTAGACTGTT | Integrase | ZP_04661979 | colonization |
pOX | TGGCGATCAATTCTTACTTT | CGTAACGAAACGAAGTAATG | phenol hydroxylase | ZP_05824220 | none |