Figure 5

Gene phylogeny and amino acid alignment of moricins. (A) Neighbor-joining (NJ) consensus tree of moricins from Galleria mellonella (Gme) and other insect species. (A) Bootstrap values next to the nodes represent the percentage of 1000 replicate trees supporting the corresponding clade. (B) Amino acid alignment of the predicted moricin peptides from Galleria together with predicted protein sequences deduced from publicly available insect sequence datasets. Species abbreviations: Spodoptera exigua (Sex), Spodoptera littoralis (Sli), Heliothis virescens (Hvi), Manduca sexta (Mse), Bombyx mori (Bmo), Plutella xylostella (Pxy), Pieris brassicae (Pbr). Sequences were aligned with MAFFT multiple alignment program. Identical residues are boxed with dark shading, and conserved residues are boxed with light shading.