Figure 6

Gloverin gene phylogeny and amino acid alignment. (A) Bootstrap values next to the nodes represent the percentage of 1000 replicate trees supporting the corresponding clade. (B) The predicted gloverin proteins from Galleria (Gme) were aligned together with other lepidopteran gloverin proteinsusing MAFFT multiple alignment program. Identical residues are boxed with dark shading, and conserved residues are boxed with light shading. Sequences Gme_gloverin2 and 5 are almost identical to the (partial) gloverin sequence deposited in Genbank (AAN15787). Abbreviated species names: Manduca sexta (Mse), Bombyx mori (Bmo), Spodoptera exigua (Sex), Helicoverpa armigera (Har), Trichoplusia ni (Tni), Plutella xylostella (Pxy).