Figure 2

Reverse-transcriptase PCR validation of gene-specific cDNA expression during embryonic stages of development. Development stage indicated by day (Days 2 through 21) post-fertilization. For determining the lengths of each amplicon, the strongest marker bands indicate fragment lengths of 500 bp (cyp19b1, foxl2b, mis, sf1, sox9a and sox9b) or 1000 bp (dax1, dax2 and foxl2a).