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Table 4 Characteristics of P. tricornutum pre-miRNA sequences.

From: Identification and characterization of microRNAs from Phaeodactylum tricornutum by high-throughput sequencing and bioinformatics analysis

miRNA name locationa mfeb lenc P-valued total reads and 5' homogeneitye hairpin characteristicsf
      PT1 PT2 PT3 bulges mismatched
pti-miR5471 chr_1:1604545:1604822:- -78.8 278 0.172 0/0 9/9 0/0 0 5
pti-miR5472 chr_10:761305:761625:- -85.9 321 0.355 64/88 23/30 145/213 0 5
pti-miR5473 chr_11:35406:35506:- -30.4 101 0.019 6/9 0 9/15 0 4
pti-miR5474 chr_15:663006:663165:+ -42.7 160 0.801 22/23 69/71 20/22 0 6
pti-miR5475 chr_18:415320:415541:- -62 222 0.44 0/0 226/230 383/385 0 4
pti-miR5476 chr_19:299730:299870:- -52.8 141 0.043 0/0 0/0 9/15 0 3
pti-miR5477 chr_1:2137385:2137503:- -26.1 119 0.025 0/0 0/0 6/9 0 6
pti-miR5478 chr_23:97534:97765:+ -76.1 232 0.029 0/0 0/0 5/7 0 4
pti-miR5479 chr_25:444580:444846:+ -79.6 267 0.1 0/0 0/0 18/19 0 4
pti-miR5480 chr_26:278601:278960:+ -105 360 0.146 0/0 0/0 6/8 0 6
pti-miR5481 chr_2:1365749:1366009:+ -70.8 261 0.213 0/0 0/0 5/5 0 4
pti-miR5482 chr_9:128204:128523:+ -101 320 0.561 0/0 0/0 5/6 0 3
pti-miR5483 chr_9:523733:524013:+ -67.6 281 0.126 0/0 0/0 10/12 0 5
  1. aIndicated the chromosome number, start and end site and sense (+) or antisense strand (-) that the pre-miRNAs located.
  2. bMinimum Free Energy (cal/mol) of pre-miRNAs, predicted by mfold.
  3. clength of pre-miRNAs.
  4. dcomputed P-values of miRNA precursors.
  5. enumber of reads that had the same 5' end as the mature miRNA and the total number reads mapped to the precursors.
  6. fasymmetric bulges larger than 2 nt and mismatched miRNA bases in the miRNA region.