Figure 3From: An RNA interference screen for identifying downstream effectors of the p53 and pRB tumour suppressor pathways involved in senescenceShRNA screen. CL3EcoR cells were transduced with ecotropic retroviruses prepared from each of the 100 pools of shRNAmirs, selected with puromycin, reseeded and shifted up to 38°C for 3 weeks. Densely growing colonies were considered to have bypassed senescence and potentially contain candidate shRNAmirs.Back to article page