An RNA interference screen for identifying downstream effectors of the p53 and pRB tumour suppressor pathways involved in senescence

Table 2 Microarray expression profiling data for common genes

Probe	Symbol	GA	p-value	HS	p-value	pRS_p53	p-value	pRS_p21	p-value	E7	p-value	E2F_DB	p-value
208832_at	ATXN10	0.36	1E-03	0.25	3E-02	-0.18	2E-01	-0.35	2E-03	0.02	9E-01	-0.23	4E-02
228080_at	LAYN	1.04	2E-04	0.30	3E-01	-0.95	1E-03	-0.70	2E-02	-0.56	6E-02	-0.11	8E-01
219922_s_at	LTBP2/3	1.32	9E-08	0.66	5E-03	-1.04	2E-05	-1.32	3E-07	-1.19	1E-06	-1.41	4E-08
223690_at	LTBP2/3	0.75	5E-09	-0.14	3E-01	-0.52	1E-05	-0.91	2E-10	-1.27	1E-14	-1.22	3E-14
204682_at	LTBP2/3	0.57	8E-04	-0.48	8E-03	-0.39	4E-02	-0.94	1E-06	-1.62	3E-12	-2.02	4E-15
227308_x_at	LTBP2/3	0.46	5E-05	0.03	9E-01	-0.43	3E-04	-0.57	4E-06	-0.28	2E-02	-0.55	4E-06
218065_s_at	TMEM9B	0.44	1E-07	-0.11	2E-01	-0.33	6E-05	-0.38	9E-06	-0.20	1E-02	-0.18	2E-02
222507_s_at	TMEM9B	0.39	2E-04	-0.19	1E-01	-0.28	1E-02	-0.34	2E-03	-0.12	3E-01	-0.07	6E-01

Log₂ fold changes in gene expression in CL3^EcoR cells upon senescence arrest are indicated as GA and upon shift up of control temperature insensitive HMF3S cells, from 34°C to 38°C, as HS respectively. Also indicated are the data obtained for these genes from CL3^EcoR cells in which senescence had been bypassed by silencing of p53 (pRS_p53) or p21^CIP1 (pRS_p21) or by inactivation of pRB using HPV16 E7 (E7) or by expression of the dominant negative E2F-DB protein.

ISSN: 1471-2164