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Table 4 B. cenocepacia K56-6 deletion mutants and their phenotype

From: Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis

Mutant name Gene(s) deleted Gene annotation Relevant experiment/microarray observation Phenotypea
K56-2ΔL1755-6 BCAL1755
BCAL1756
Hypothetical protein
Phosphohydrolase
Upregulated in the presence of amikacin, in J2315-A and in BCC1617 None observed
K56-2ΔM0831 BCAM0831 ABC transporter Upregulated in the presence of amikacin None observed
K56-2ΔS0293-2 BCAS0292
BCAS0293
Hypothetical protein
Nematocidal protein AidA
Upregulated in the presence of TMP/SMX None observed
K56-2ΔM0924 BCAM0924 Response regulator protein Upregulated in the presence of chlorpromazine None observed
K56-2ΔM0925-7 BCAM0925
BCAM0296
BCAM0297
Multidrug efflux system OMP
Multidrug efflux system protein
Multidrug efflux system protein
Upregulated in the presence of chlorpromazine Increased susceptibility for chlorhexidine (14 μg/ml, wt 22 μg/ml)
Increased susceptibility for azithromycin (100 μg/ml, wt 150 μg/ml)
Decreased growth rate in the presence of 1.0 mM chlorpromazine (μ = 0.49, wt μ = 0.55)
K56-2ΔM2186-8 BCAM2186
BCAM2187
BCAM2188
Efflux system transport protein
ABC-type efflux carrier protein
Outer membrane efflux protein
Upregulated in the presence of chlorpromazine None observed
K56-2ΔS0167 BCAS0167 Squalene-hopene cyclase Upregulated in the presence of chlorpromazine Increased susceptibility for chlorhexidine (13 μg/ml, wt 22 μg/ml)
Decreased growth rate in the presence of 1.0 mM chlorpromazine (μ = 0.62, wt μ = 0.80)
K56-2ΔS0081 BCAS0081 ABC transporter Upregulated in outbreak strains BCC1616 and BCC1617 Increased susceptibility for chlorhexidine (14 μg/ml, wt 22 μg/ml)
Increased susceptibility for tetracycline (100 μg/ml, wt 150 μg/ml)
  1. a The following antibiotics and antimicrobials were tested on all mutants, except K56-2ΔS0167: amikacin, tobramycin, meropenem, ceftazidime, trimethoprim, azithromycin, erythromycin, levofloxacin, tetracycline, chloramphenicol, chlorhexidine