Table 4 B. cenocepacia K56-6 deletion mutants and their phenotype
Mutant name | Gene(s) deleted | Gene annotation | Relevant experiment/microarray observation | Phenotypea |
|---|---|---|---|---|
K56-2ΔL1755-6 | BCAL1755 BCAL1756 | Hypothetical protein Phosphohydrolase | Upregulated in the presence of amikacin, in J2315-A and in BCC1617 | None observed |
K56-2ΔM0831 | BCAM0831 | ABC transporter | Upregulated in the presence of amikacin | None observed |
K56-2ΔS0293-2 | BCAS0292 BCAS0293 | Hypothetical protein Nematocidal protein AidA | Upregulated in the presence of TMP/SMX | None observed |
K56-2ΔM0924 | BCAM0924 | Response regulator protein | Upregulated in the presence of chlorpromazine | None observed |
K56-2ΔM0925-7 | BCAM0925 BCAM0296 BCAM0297 | Multidrug efflux system OMP Multidrug efflux system protein Multidrug efflux system protein | Upregulated in the presence of chlorpromazine | Increased susceptibility for chlorhexidine (14 μg/ml, wt 22 μg/ml) Increased susceptibility for azithromycin (100 μg/ml, wt 150 μg/ml) Decreased growth rate in the presence of 1.0 mM chlorpromazine (μ = 0.49, wt μ = 0.55) |
K56-2ΔM2186-8 | BCAM2186 BCAM2187 BCAM2188 | Efflux system transport protein ABC-type efflux carrier protein Outer membrane efflux protein | Upregulated in the presence of chlorpromazine | None observed |
K56-2ΔS0167 | BCAS0167 | Squalene-hopene cyclase | Upregulated in the presence of chlorpromazine | Increased susceptibility for chlorhexidine (13 μg/ml, wt 22 μg/ml) Decreased growth rate in the presence of 1.0 mM chlorpromazine (μ = 0.62, wt μ = 0.80) |
K56-2ΔS0081 | BCAS0081 | ABC transporter | Upregulated in outbreak strains BCC1616 and BCC1617 | Increased susceptibility for chlorhexidine (14 μg/ml, wt 22 μg/ml) Increased susceptibility for tetracycline (100 μg/ml, wt 150 μg/ml) |