Skip to main content

Table 2 HPLC-MS/MS analysis of seed coat extracts of black (iRT) and brown (irT) soybean

From: Combined analysis of transcriptome and metabolite data reveals extensive differences between black and brown nearly-isogenic soybean (Glycine max) seed coats enabling the identification of pigment isogenes

Compound Identity [M+] (m/z) Major fragment(s) Rt (min) Genotypea
1 Unknown 1 413 183, 115 1.4 B
2 Unknown 2 381 184, 125 1.6 irT
3 Unknown 3 183 - 1.7 iRT
4 Unknown 4 162 - 1.9 B
5 Unknown 5 409 303 1.9 B
6 Unknown 6 437 273 2.3 B
7 Unknown 7 416 - 2.3 B
8 Unknown 8 580 182, 265, 437 2.5 B
9 Unknown 9 507 485, 323, 132 2.9 B
10 Orcinol O-hexoside 305 287 4.0 irT
11 Unknown 10 331 248 4.2 irT
12 Procyanidin trimer 1 867 579, 309, 299 4.7 iRT
13 Procyanidin B2* 579 291 5.5 B
14 Unknown 11 607 409, 455 6.0 B
15 Procyanidin trimer 2 867 577, 407 6.4 B
16 Delphinidin-3-O-glucoside* 465 303 6.6 iRT
17 Procyanidin trimer 3 867 579 6.6 iRT
18 Epicatechin* 291 - 6.9 B
19 Cyanidin-3-O-galactoside* 449 287 7.1 iRT
20 Daidzein O-hexoside malonylated 503 417 7.4 irT
21 Unknown 12 481 319 7.4 irT
22 Cyanidin-3-O-glucoside* 449 287 7.5 iRT
23 Petunidin-3-O-glucoside* 479 319, 303 8.0 iRT
24 Unknown 13 341 278, 111 8.1 irT
25 Pelargonidin-3-O-glucoside* 433 - 8.3 iRT
26 Peonidin-3-O-glucoside* 463 301, 286 8.7 iRT
27 Procyanidin dimer 2 579 409, 291 9.2 B
28 Unknown flavonol 1 331 - 9.0 irT
29 Procyanidin trimer 4 867 579, 699 9.2 iRT
30 A-type procyanidin dimer 593 - 9.7 iRT
31 Unknown anthocyanin 1 317 - 9.6 iRT
32 Unknown flavonol 2 615 - 9.6 irT
33 Cyanidin* 287 - 10.0 iRT
34 Unknown 14 487 - 10.8 B
35 Diosmetinidin* 285 - 10.3 iRT
36 Unknown benzoic acid 1 633 331, 315 11.0 irT
37 Quercetin-3-O-glucoside* 465 303 11.0 Bb
  1. *Based on co-chromatographic and MS fragamentation comparison to an authentic standard.
  2. aBlack soybean (iRT), brown soybean (irT), both (B).
  3. bTrace amounts in iRT.