Skip to main content

Table 1 Distribution of microsatellites GSSRs (BSSRs), and ESSRs) of carrot*

From: Microsatellite isolation and marker development in carrot - genomic distribution, linkage mapping, genetic diversity analysis and marker transferability across Apiaceae

 

GSSRs

BSSRs

ESSRs

SSR type

Count (%)

Mean # of repeat units/mean SSR length (bp)

Most frequent motifs‡

Count (%)

Mean # of repeat units/mean SSR length (bp)

Densityǂ(SSR/Mb)

Most frequent motifs‡

Count (%)

Mean # of repeat units/mean SSR length (bp)

Densityǂ(SSR/Mb)

Most frequent motifs‡

Dinucleotide

116 (46.8)

10.0/21.2

AG(61), AC(34)

39 (16.7)

7.8/15.5

22.4

AT(46), AC, AG

207 (25.2)

8.1/16.5

54.1

AG(78), AC

Trinucleotide

28 (11.3)

7.0/21.3

AAC, AGT, AAG

97 (41.5)

4.3/12.9

55.7

AAG(27), AAC(17), AGT(13), ACT(12), AAG(27)

411 (50.1)

4.4/13.1

107.5

AAG(25), ACT(15), AGG(10), AGC(10)

Tetranucleotide

98 (39.5)

5.9/26.2

ACAT (59), AAAC

57 (24.4)

3.3/12.2

32.8

AAAT(33), AATT(21)

129 (15.7)

3.1/12.3

33.7

AAAG, AAAT

Pentanucleotide

2 (0.8)

3.0/15.0

-

27 (11.5)

3.0/15.2

15.5

AAAAG, AGCCG

29 (3.5)

3.1/15.7

7.6

AGCCC

Hexanucleotide

2 (0.8)

3.0/18.0

-

7 (3.0)

3.0/18.0

4.0

-

39 (4.8)

3.2/18.9

10.2

AAAAAG, AAAGAG

Heptanucleotide

0 (0)

3.0/21.0

-

4 (1.7)

3.0/21.0

2.3

-

4 (0.5)

3.0/21.0

1.0

-

Octanucleotide

2 (0.8)

3.0/24.0

-

3 (1.3)

3.0/24.0

1.7

-

2 (0.2)

3.5/28.0

0.5

-

Total perfect SSRs

248 (100)

7.9/23.1

 

234 (100)

4.4/13.9

134.5

 

821 (100)

5.0/14.2

214.8

 

Compound SSRs

41 (16.5)

-/49.9

 

6 (2.6)

-/38.3

3.4

 

26 (3.1)

-/32.9

6.8

 

Total seq. (Mbp)ζ

0.04

  

1.74

   

3.82

   

GC content (%)

37.9

  

38.3

   

42.3

   
  1. * A minimum of 6 repeat units (r.u.) for dinucleotides, 4 r.u. for trinucleotides, and 3 r.u. for tetra-, penta-, hexa- hepta- and octanucleotides were used as parameters for searching microsatellites in genomic and EST sequence of carrot. ǂ Only density values for SSRs from BAC end sequences (BSSRs) and from ESTs (ESSRs) are presented (because GSSRs derive from an SSR-enriched library, analyses of this dataset would result in an overestimation of the SSR density in genomic DNA). ‡ SSR motif considering complementary (e.g., "AAG" includes AAG + CTT motifs); SSR motifs occurring at a rate of at least 5% of the total are listed with occurrence as percentage within each SSR type class in parentheses; SSR classes without predominance of a particular motif are denoted as "-". ζ Total length of sequences analyzed. γ Includes both GSSRs and BSSRs.