Table 1 Oligonucleotide primers used for RT-qPCR analysis.
Description | CitEST | Forward/Reverse | Amp | |
|---|---|---|---|---|
Selected genes to validate the microarray | ||||
LEA | Lea 5 | CAS-PT-303903 | TCGGACTGGTATCATGGA/GTAGTACCCAGTGATGGGA | 99 |
MIR | miraculin | CAS-CR-215276 | AGCCCTGTAATGAAGAACC/TAGCAACGTTTCAGCTCC | 100 |
TIR | TIR-NBS-LRR | CAS-CS-103511 | CATGATGAGGACGTGGG/AAGTGATCCGACTCGAC | 103 |
RPS4 | Disease resistance RPS4 | CAS-PT-300852 | CCAAGATCTTGAATATCTTCCC/GCAAGTTGAGCTCAATTAGG | 112 |
UNC | unclassified proteins | CAS-PT-310250 | TCTCTTGTTCTTCATGCAGT/TATGCATCTTGCCTTCATTC | 116 |
Selected endogenous control genes | ||||
ETEF2 | eukaryotic translation elongation factor 2 | CAS-PT-306679 | TTGAGGCTTCTGAATCGAG/CTTTCCAGATGAACCTCTCC | 97 |
EGIDH | NADP-isocitrate dehydrogenase | CAS-CS-112964 | CATTGAACATGCAGTTGAGG/ATTCTCATGACGTGTCGG | 91 |
CYC | cyclophilin | CAS-PT-301486 | AGAGTATGCAGAGGAATGG/GTCCTTAACAGAAGTCCGT | 107 |
UBQ | ubiquitin | CAS-PT-300961 | TTCGTCAGTTGACTAATCCT/GTTGCTGTGTTGACTGTG | 95 |
TUB | tubulin | - | TTTGTAAGATCCCTCCGA/TCACCCTCCTGAACATTT | 87 |