Figure 2From: Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomesRepresentative examples of yeast mutants with increased levels of Slt2 phosphorylation. Exponential phase cultures of the indicated yeast strains were taken and processed for immunoblotting as described under "Methods". Western blots detecting the phosphorylated form of Slt2 (P-Slt2) and actin as loading control are shown. Numbers correspond to the P-Slt2 fold-change obtained from densitometric quantification of the P-Slt2 bands from Western blots normalized with respect to the actin bands, using the values of the wild-type strain as reference (fold-change set to 1.0).Back to article page