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Figure 5 | BMC Genomics

Figure 5

From: Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes

Figure 5

Slt2 activation in gat2 Δ and lea1 Δ mutants is not transmitted via Rom2 nor related to variations in chitin content. (a) Analysis of chitin content of gat2 Δ and lea1 Δ cells after Calcofluor white staining by means of flow cytometry as described in Figure 4. (b) Western blots detecting Phospho-Slt2 (P-Slt2) and actin as loading control in total protein extracts from single (gat2 Δ and lea1 Δ) and double (lea1 Δrom 2Δ, gat2 Δrom2 Δ, lea1 Δbck1 Δ and gat2 Δbck 1Δ) mutant strains.

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