Examination of transcriptional activation and Slt2 phosphorylation in mutants selected from the screening. Bars correspond to levels of MLP1-lacZ (a) and CWP1-lacZ (b) expression observed in the indicated deletion mutants and the wild-type strain. Three independent experiments were carried out to calculate the means of β-galactosidase activity and standard deviations. The line graph represents the fold-change in Slt2 phosphorylation for each mutant relative to the values observed in the wild-type strain (1x) as described in Table 1. 2D scatter plots of fold-change in phospho-Slt2 vs transcriptional activation are shown on the left side, including the Pearson's correlation coefficient (r).