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Table 1 S. cerevisiae mutants with increased levels of dually phosphorylated MAP Kinase Slt2

From: Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes

ORF Gene P-Slt2 Functional Group Description
YAL058W CNE1 16.4 Cell wall and morphogenesis Calnexin 1. may be involved in a QC process for secretory pathway proteins
YBL007C SLA1 8.1 Cell wall and morphogenesis Functions in the assembly of cortical actin cytoskeleton
YBR078W ECM33 14.4 • Cell wall and morphogenesis GPI-anchored protein required for CW integrity and mannoprotein biosynthesis
YCR088W ABP1 2.3 Cell wall and morphogenesis Actin binding protein that functions in clathrin- and actin-mediated endocytosis
YDL095W PMT1 10.8 Cell wall and morphogenesis Mannosyltransferase, first step in O-glycosylation
YDR349C YPS7 16.6 • Cell wall and morphogenesis GPI-anchored aspartyl protease involved in maintaining cell wall integrity
YJL062W LAS21 3.6 Cell wall and morphogenesis Protein required for addition of a side chain to the GPI core structure
YJL099W CHS6 2.9 Cell wall and morphogenesis Protein involved in chitin synthase Chs3p activity
YJR075W HOC1 2.1 • Cell wall and morphogenesis Alpha-1,6-mannosyltransferase activity
YLR319C BUD6 2.4 Cell wall and morphogenesis Budding 6, required for bipolar budding and involved in bud site selection
YLR337C VRP1 8.6 Cell wall and morphogenesis Proline-rich protein verprolin, involved in cytoskeletal organization
YLR350W ORM2 2.6 Cell wall and morphogenesis Putative transmembrane protein that may function in the CWI pathway
YLR370C ARC18 5.5 Cell wall and morphogenesis Component of the ARP2/3 actin-organizing complex, involved in actin assembly
YMR307W GAS1 27.4 • Cell wall and morphogenesis GPI-anchored protein with 1,3-beta-glucanosyltransferase activity
YNL079C TPM1 7.2 • Cell wall and morphogenesis Tropomyosin, functions in a variety of processes involving the actin cytoskeleton
YNL116W DMA2 3.4 Cell wall and morphogenesis Functions in spindle positioning and septin ring assembly
YNL192W CHS1 2.0 Cell wall and morphogenesis Chitin synthase I, has a repair function during cell separation
YOR002W ALG6 6.3 Cell wall and morphogenesis Dolichyl-phosphate-mannose-protein mannosyltransferase activity
YER037W PHM8 4.0 Metabolism Lysophosphatidic acid phosphatase
YJL137C GLG2 2.3 Metabolism Transferase activity, transferring hexosyl groups
YHL023C RMD11 2.9 Nuclear Protein possibly involved in meiotic nuclear division
YPR031W NTO1 2.0 Nuclear May be involved in chromatin-mediated transcription regulation
YBR101C FES1 2.8 Protein metabolism NEF for the cytosolic chaperone Ssa1p. Response to oxidative stress
YDR069C DOA4 2.6 Protein metabolism Ubiquitin-specific protease that acts in recycling ubiquitin
YFL007W BLM10 4.0 Protein metabolism Proteasome assembly
YBL024W NCL1 5.8 RNA metabolism tRNA (cytosine-5-)-methyltransferase activity
YPL029W SUV3 2.0 RNA metabolism Mitochondrial RNA helicase of the DEAD box family
YPL213W LEA1 9.0 RNA metabolism RNA splicing factor activity
YDL047W SIT4 4.3 Signal transduction Serine/threonine phosphatase involved in cell cycle regulation/ion homeostasis
YDR162C NBP2 16.9 Signal transduction Negative regulator of the HOG pathway
YDR389W SAC7 7.5 Signal transduction GTPase-activating protein for Rho1p
YER155C BEM2 2.4 • Signal transduction GTPase-activating (GAP) protein that regulates Rho1p
YHR082C KSP1 2.9 Signal transduction Serine/threonine kinase involved in filamentous growth
YHR206W SKN7 2.5 Signal transduction Transcription factor involved in the oxidative and osmotic stress responses
YJR074W MOG1 5.4 Signal transduction Involved in nuclear protein import, plays a role in osmoregulation via SLN1-SKN7
YKL126W YPK1 2.3 Signal transduction Putative S/T protein kinase possibly related with the CWI and sphingolipids
YLR371W ROM2 2.5 Signal transduction GDP-GTP exchange factor for Rho1p
YNL053W MSG5 5.4 Signal transduction Dual-specificity protein tyrosine phosphatase involved in response to pheromone
YNR047W FPK1 3.9 Signal transduction Serine/threonine protein kinase that regulates phospholipid asymmetry
YLR242C ARV1 8.6 Sphingolipid metabolism Protein involved in sterol uptake required for normal sphingolipid metabolism
YER139C RTR1 5.2 Transcription Protein required for growth at high temperature, RNA polymerase II factor
YMR136W GAT2 10.7 Transcription GATA zinc finger transcription factor
YNL025C SSN8 2.2 Transcription RNA polymerase II transcription mediator
YPL042C SSN3 3.9 Transcription Cyclin-dependent serine/threonine protein kinase of the RNA polymerase II
YPR065W ROX1 4.7 Transcription Transcriptional repressor of hypoxic genes
YPR115W   3.6 Transcription Phosphoinositide binding. Response to oxidative stress
YDR200C VPS64 3.9 Transport Class B vacuolar sorting protein involved in Prc1p trafficking/α-factor secretion
YFL048C EMP47 4.1 Transport Golgi and ER membrane protein involved in glycoprotein secretion
YGR028W MSP1 2.1 Transport Intra-mitochondrial sorting protein, member of the AAA family of ATPases
YHR032W ERC1 3.5 Transport Member of the MatE family. Transporter activity
YJL133W MRS3 5.2 Transport Mitochondrial carrier (MCF, family of membrane transporters). Iron transport
YJR152W DAL5 5.7 Transport Member of the allantoate family of the major facilitator superfamily (MFS)
YLR292C SEC72 2.9 Transport Component of ER protein-translocation subcomplex.
YCR090C   2.0 Unknown Unknown function
YDL173W   4.1 Unknown Unknown function
YDR290W   4.2 Unknown Unknown function
YGR022C   3.9 Unknown Unknown function
YLR250W SSP120 5.2 Unknown Unknown function
YLR338W   7.2 Unknown Unknown function
YMR119W-A   3.2 Unknown Unknown function
YNL058C   3.0 Unknown Unknown function
YNL105W   5.5 Unknown Unknown function
YNR014W   2.5 Unknown Unknown function
YPL158C   3.0 Unknown Unknown function
  1. Levels of Slt2 phosphorylation (P-Slt2) correspond to the fold-change in each mutant strain relative to the wild-type calculated as described in Methods. Representative data of at least replicated experiments are shown. Functional categories and descriptions were assigned based on the information provided by the BIOBASE Knowledge Library Proteome. Genes were grouped together on the basis of their functional category. Those gene deletions that have been reported to be synthetic lethal with slt2 Δ are labeled with a black dot.