Table 1 Summary of IVT and WG-DASL Methods
From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay
| IVT | WG-DASL | |
|---|---|---|
|
Target Preparation
Protocol Name | In Vitro Transcription |
cDNA-mediated annealing, selection, extension and ligation |
|
Total RNA Input
Amount | 50-100 ng | 10-200 ng |
| Priming Method |
Reverse Transcription off polyA tail |
Poly(T) and random priming with biotinylated nonamers |
| Amplification | In Vitro Transcription (Linear) | PCR (Exponential) |
| Hybridization | Illumina BeadChip | |
