Table 1 Summary of IVT and WG-DASL Methods
From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay
| Â | IVT | WG-DASL |
|---|---|---|
Target Preparation Protocol Name | In Vitro Transcription | cDNA-mediated annealing, selection, extension and ligation |
Total RNA Input Amount | 50-100 ng | 10-200 ng |
Priming Method | Reverse Transcription off polyA tail | Poly(T) and random priming with biotinylated nonamers |
Amplification | In Vitro Transcription (Linear) | PCR (Exponential) |
Hybridization | Illumina BeadChip | |