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Table 1 Summary of IVT and WG-DASL Methods

From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay

  IVT WG-DASL
Target Preparation
Protocol Name
In Vitro Transcription cDNA-mediated annealing,
selection, extension and
ligation
Total RNA Input
Amount
50-100 ng 10-200 ng
Priming Method Reverse Transcription off
polyA tail
Poly(T) and random priming
with biotinylated nonamers
Amplification In Vitro Transcription (Linear) PCR (Exponential)
Hybridization Illumina BeadChip