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Table 2 RNA quality as assessed by 260/280 ratio

From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay

Sample ID Before Amplification Amplified 1st Globin Reduction 2nd Globin Reduction Pooled Globin Reduction Diluted to 20 ng/ul Average
C00023 (tRNA) 2.03 2.01     2.07 2.04
C00023 (GC RNA) 1.98 1.99 2.02 1.89 2.00 1.94 1.97
C00027 (tRNA) 2.06 2.01     2.06 2.04
C00027 (GC RNA) 1.98 2.01 2.02 1.95 1.94 1.89 1.97
C00169 (tRNA) 2.04 2.12     1.89 2.02
C00169 (GC RNA) 1.96 1.95 1.88 1.90 2.02 1.84 1.93
C00179 (tRNA) 2.04 1.99     2.12 2.05
C00179 (GC RNA) 1.95 1.98 1.90 2.01 2.01 2.02 1.98
C00275 (tRNA) 2.03 1.95     2.14 2.04
C00275 (GC RNA) 1.99 1.99 1.87 1.91 1.93 2.01 1.95
C00304 (tRNA) 2.05 1.99     2.25 2.10
C00304 (GC RNA) 1.98 2.00 1.89 2.11 1.91 1.96 1.98
C00311 (tRNA) 2.04 2.10     2.12 2.09
C00311 (GC RNA) 2.02 2.00 2.04 1.93 1.97 2.01 2.00
C00342 (tRNA) 2.01 2.07     2.21 2.10
C00342 (GC RNA) 2.03 2.03 1.97 1.99 1.93 1.97 1.99
  1. RNA quality was assessed before and after globin reduction as well as before and after amplification. tRNA: total RNA; GC RNA: GLOBINclear treated RNA or globin reduced RNA.