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Table 2 RNA quality as assessed by 260/280 ratio

From: Gene expression profiling of human whole blood samples with the Illumina WG-DASL assay

Sample ID

Before Amplification

Amplified

1st Globin Reduction

2nd Globin Reduction

Pooled Globin Reduction

Diluted to 20 ng/ul

Average

C00023 (tRNA)

2.03

2.01

   

2.07

2.04

C00023 (GC RNA)

1.98

1.99

2.02

1.89

2.00

1.94

1.97

C00027 (tRNA)

2.06

2.01

   

2.06

2.04

C00027 (GC RNA)

1.98

2.01

2.02

1.95

1.94

1.89

1.97

C00169 (tRNA)

2.04

2.12

   

1.89

2.02

C00169 (GC RNA)

1.96

1.95

1.88

1.90

2.02

1.84

1.93

C00179 (tRNA)

2.04

1.99

   

2.12

2.05

C00179 (GC RNA)

1.95

1.98

1.90

2.01

2.01

2.02

1.98

C00275 (tRNA)

2.03

1.95

   

2.14

2.04

C00275 (GC RNA)

1.99

1.99

1.87

1.91

1.93

2.01

1.95

C00304 (tRNA)

2.05

1.99

   

2.25

2.10

C00304 (GC RNA)

1.98

2.00

1.89

2.11

1.91

1.96

1.98

C00311 (tRNA)

2.04

2.10

   

2.12

2.09

C00311 (GC RNA)

2.02

2.00

2.04

1.93

1.97

2.01

2.00

C00342 (tRNA)

2.01

2.07

   

2.21

2.10

C00342 (GC RNA)

2.03

2.03

1.97

1.99

1.93

1.97

1.99

  1. RNA quality was assessed before and after globin reduction as well as before and after amplification. tRNA: total RNA; GC RNA: GLOBINclear treated RNA or globin reduced RNA.
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BMC Genomics

ISSN: 1471-2164

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