Figure 3

Gene expression profiles. Relative quantification throughout early follicular development and in the two follicular compartments (n = 3-4 except for Ant, n = 2). Profiles showed a significant increase in gene expression for BMP15 and AMH from the primary and secondary stage, respectively. By contrast, KL and VASA genes were expressed at all stages from the primordial follicular stage on, with no significant differential expression during the process of early folliculogenesis. The Y axis corresponds to the relative expression normalized by 2 reference genes (Actin β and RPL19). The X axis corresponds to the different follicular stages of the 2 follicular compartments. Abbreviations: Pd: primordial, Pm: primary, Sec: secondary, SA: small antrum. *< 0.05, **< 0.01.