Evaluation of two commercial global miRNA expression profiling platforms for detection of less abundant miRNAs

BMC Genomics

Table 1 The number of mismatches to homologous synthetic miRNAs impacts assay cross-reaction tendency¤.

	miRCURY					TaqMan
Number of nucleotide mismatches to synthetic miRNA*	0	1	2	3	4	0	1	2	3	4
Number of assays§	3	18	34	52	60	3	18	30	45	57
Number of false positive assays (n)#	3	17	25	38	40	2	15	22	26	33
False positive rate (false positives/number of assays) (%)	100	94	74	73	67	67	83	73	58	58

¤ A given assay can be homologous to more than one synthetic miRNA and thereby appear in more than one of the mismatch categories. This may cause the false positive rates to be over estimated.
* 0 mismatches means that only the length differs between the synthetic miRNA and the miRNA assayed.
§Number of assays with the given number of nucleotide mismatches to a miRNA in the synthetic samples.
#An assay was scored as false positive if detected at Ct < 38 (miRCURY) or Ct < 30 (TaqMan) in one or more of the synthetic samples #1 and #2.

ISSN: 1471-2164