Table 3 Assessment of sensitivity and reproducibility at different miRNA input levels, spanning five orders of magnitude, Pearson correlation of duplicate measurements§.
The 125 spike-in miRNAs for which both platforms have assays | ||
|---|---|---|
miRCURY | TaqMan | |
miRNA copies pr. PCR amplification | r (n detected) | r (n detected) |
5,000/20,000 | 0.995 (124) | 0.981 (123) |
500/2,000 | 0.901 (124) | 0.853 (121) |
0.990 (116)* | ||
50/200 | 0.920 (119) | 0.930 (101) |
5/20 | 0.829 (93) | 0.843 (59) |
0.5/2 | 0.228 (35) | 0.249 (34) |