Table 1 Bacterial strains and plasmids used in this study.

Strains

P. aeruginosa

PAO1

wild type

T. Köhler

ΔpppA-ppkA

PAO1 with unmarked deletion of pppA-ppkA

This work

PAO1::tn7TLAC

PAO1::mini-tn7T-LAC

This work

Δ::tn7TLAC

ΔpppA-ppkA:: mini-tn7T-LAC

This work

Δ::tn7TLACpak

ΔpppA-ppkA:: mini-tn7T-LAC-pppA-ppkA

This work

E. coli

XL1-blue

rec A1 end A1 gyr A96 thi hsd R17(r_k^-, m_k⁺) sup E44 rel A1 lac [F' pro AB⁺ lac Iq Δ(lac Z)M15::Tn10]

Stratagene

JM109

end A1 rec A1 gyr A96 thi hsd R17 (rk^-mk⁺) sup E44 Δ(lac-pro AB), [F' tra D36 pro AB lac Iq Δ(lac Z)M15]

Promega

Plasmids

pEX18Ap

Ap^R, ori T⁺ sac B⁺, gene repacement vector with MSC from pUC18

(24)

pPS858

Ap^R, Gm^R, gfp⁺, contains Gm^R-GFP FRT cassette

(24)

pFLP2

Ap^R, contains FLP recombinase gene

(24)

pUC18-mini-Tn7T-LAC

Ap^R, Gm^R, mini-Tn7T-Gm Gateway-compatible cloning and delivery vector with lacI^q-Ptac expression cassette

(7)

pTNS2

Helper plasmid for integration of the expression casette

(7)

pUC18miniTn7TLACpak

pUC18-mini-Tn7T-LAC expressing pppA-ppkA

This work