|
Strains
| | |
|
P. aeruginosa
| | |
|
PAO1
|
wild type
|
T. Köhler
|
|
ΔpppA-ppkA
|
PAO1 with unmarked deletion of pppA-ppkA
|
This work
|
|
PAO1::tn7TLAC
|
PAO1::mini-tn7T-LAC
|
This work
|
|
Δ::tn7TLAC
|
ΔpppA-ppkA:: mini-tn7T-LAC
|
This work
|
|
Δ::tn7TLACpak
|
ΔpppA-ppkA:: mini-tn7T-LAC-pppA-ppkA
|
This work
|
|
E. coli
| | |
|
XL1-blue
|
rec A1 end A1 gyr A96 thi hsd R17(rk-, mk+) sup E44 rel A1 lac [F' pro AB+ lac Iq Δ(lac Z)M15::Tn10]
|
Stratagene
|
|
JM109
|
end A1 rec A1 gyr A96 thi hsd R17 (rk-mk+) sup E44 Δ(lac-pro AB), [F' tra D36 pro AB lac Iq Δ(lac Z)M15]
|
Promega
|
|
Plasmids
| | |
|
pEX18Ap
|
ApR, ori T+ sac B+, gene repacement vector with MSC from pUC18
|
(24)
|
|
pPS858
|
ApR, GmR, gfp+, contains GmR-GFP FRT cassette
|
(24)
|
|
pFLP2
|
ApR, contains FLP recombinase gene
|
(24)
|
|
pUC18-mini-Tn7T-LAC
|
ApR, GmR, mini-Tn7T-Gm Gateway-compatible cloning and delivery vector with lacIq-Ptac expression cassette
|
(7)
|
|
pTNS2
|
Helper plasmid for integration of the expression casette
|
(7)
|
|
pUC18miniTn7TLACpak
|
pUC18-mini-Tn7T-LAC expressing pppA-ppkA
|
This work
|
