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Table 2 Production of virulence factors by PAO1 and ΔpppA-ppk A and MIC profiles

From: A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence

  Strain
  PAO1 Δ pppA - ppk A
Virulence factor 24 h 48 h 72 h 24 h 48 h 72 h
elastases - elastine degradation + + + + + +
elastases - casein degradation    +    +
pyocyanin + + + + + +
pyoverdine (OD405/OD600)a 0.68 (± 0.032) 0.39 (± 0.054)
phospholipase C +    +   
swarming motility +    +   
swimming motility +    +   
twitching motility    +    +
alginate   +    +  
MIC (μg•ml -1 ) b PAO1 Δ pppA - ppk A
chloramphenicol 25 55
carbenicillin 35 75
  1. + sign indicates positive reaction or manifestation
  2. a The pyoverdine values are expressed as OD405 values of culture supernatants normalized to cell density at OD600. Averages based on three independent assays are presented. The numbers in the parentheses represents standard errors of the means. The differences between the mutant and wild-type strain are statistically significant (P < 0.05).
  3. b The data are representative of four replicates.