Table 4 Transcript level comparison of P.aeruginosa genes between microarray analysis and real-time PCR analysis
non-stress conditions | oxidative stress conditions | ||||
|---|---|---|---|---|---|
| Â | mRNA level changea | Â | mRNA level changea | ||
Gene (name) | microarray | real-time PCR | Gene (name) | microarray | real-time PCR |
PA2570 (pa1L) | 2.47 | 2.71 (± 0.45) | PA4764 (fur) | -2.44 | -1.66 (± 0.04) |
PA4296 (pprB) | 3.69 | 3.71 (± 0.39) | PA4227 (pchR) | 2.47 | 1.60 (± 0.23) |
PA4175 (prpL) | 4.65 | 3.63 (± 0.38) | PA2384 | 3.11 | 2.01 (± 0.15) |
PA3724 (lasB) | 2.23 | 2.98 (± 0.27) | PA1317 (cyoA) | 3.12 | 3.00 (± 0.27) |
PA3622 (rpoS) | 3.86 | 4.4 (± 0.45) | PA4471 (fagA) | 13.62 | 7.51 (± 0.61) |
PA0996 (pqsA) | 4.56 | 2.74 (± 0.12) | PA3617 (recA) | -2.26 | -1.54 (± 0.04) |
PA1001 (phnA) | 2.54 | 1.51 (± 0.19) | PA3007 (lexA) | -2.02 | -1.98 (± 0.02) |
PA1317 (cyoA) | -3.08 | -2.59 (± 0.08) | PA5360 (phoB) | -2.00 | -1.82 (± 0.06) |
PA1754 (cysB) | 1.73 | 2.75 (± 0.19) | PA2165 | 3.96 | 3.45 (± 0.4) |
PA1127 | -2.94 | -5.00 (± 0.04) | PA0073 (tagT1) | 44.76 | 29.55 (± 3.92) |
PA4139 | 12.71 | 13.3 (± 0.71) | PA0072 (tagS1) | 5.15 | 3.67 (± 0.7) |