Skip to main content

Table 4 Transcript level comparison of P.aeruginosa genes between microarray analysis and real-time PCR analysis

From: A eukaryotic-type signalling system of Pseudomonas aeruginosa contributes to oxidative stress resistance, intracellular survival and virulence

non-stress conditions oxidative stress conditions
  mRNA level changea   mRNA level changea
Gene (name) microarray real-time PCR Gene (name) microarray real-time PCR
PA2570 (pa1L) 2.47 2.71 (± 0.45) PA4764 (fur) -2.44 -1.66 (± 0.04)
PA4296 (pprB) 3.69 3.71 (± 0.39) PA4227 (pchR) 2.47 1.60 (± 0.23)
PA4175 (prpL) 4.65 3.63 (± 0.38) PA2384 3.11 2.01 (± 0.15)
PA3724 (lasB) 2.23 2.98 (± 0.27) PA1317 (cyoA) 3.12 3.00 (± 0.27)
PA3622 (rpoS) 3.86 4.4 (± 0.45) PA4471 (fagA) 13.62 7.51 (± 0.61)
PA0996 (pqsA) 4.56 2.74 (± 0.12) PA3617 (recA) -2.26 -1.54 (± 0.04)
PA1001 (phnA) 2.54 1.51 (± 0.19) PA3007 (lexA) -2.02 -1.98 (± 0.02)
PA1317 (cyoA) -3.08 -2.59 (± 0.08) PA5360 (phoB) -2.00 -1.82 (± 0.06)
PA1754 (cysB) 1.73 2.75 (± 0.19) PA2165 3.96 3.45 (± 0.4)
PA1127 -2.94 -5.00 (± 0.04) PA0073 (tagT1) 44.76 29.55 (± 3.92)
PA4139 12.71 13.3 (± 0.71) PA0072 (tagS1) 5.15 3.67 (± 0.7)
  1. a Fold changes represent the ratio of the expression levels in the comparison of P. aeruginosa ΔpppA-ppkA and wild type PAO1. Minus (-) sign indicates a decreased expression in ΔpppA-ppkA mutant strain.