Figure 4

FISH analysis using turkey BAC probes shows an inversion of 0.7 Mb between MGA16 and GGA14. CHORI-260 BAC 110H19 exhibits split signals (green arrows) in chicken, (A) leptotene stage nucleus and (C) pachytene bivalent FISH, as predicted by its widely spread and same strand BES matches to the chicken sequence (WUGSC2.1/galGal3 14315000-14844336). The FISH signal from CHORI-260 BAC 094E20 (red arrows, WUGSC2.1/galGal3 14715031-14890809) aligns consistently with an unbroken sequence mostly internal to the two 110H19 ends and overlapping with one of them on a (B) leptotene stage nucleus (same cell as A) and (D) pachytene (same bivalent as C). (E) Diagram of the inversion between MGA16 and GGA14 based on FISH analysis, overgo hybridization and BES alignments. Chicken sequence coordinates of the BACs are as shown. Asterisks: BES aligned too far apart and to the same DNA strand in the chicken sequence for CHORI-260 110H19 and 106P19. A, B: scale bar = 5 μm; C, D: scale bar = 1 μm.