Figure 1

Morphology and surface marker presentation of human MSC (A-C). Human mesenchymal stem cells (MSC) were isolated from bone marrow aspirates. During primary culture (A) and up to passage 3 (B) they showed a typical fibroblast-like morphology and grew in a swirling-like pattern. Flow cytometry analysis demonstrated a homogenous MSC population (C). As expected, MSC were negative for reactivity to antigens CD14, CD34 and CD45, but positive for reactivity to antigens CD44, CD73, CD90, CD105 and CD166.