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Table 3 Real-time PCR validation of selected genes identified by microarray analysis as differentially regulated

From: Transcriptional responses of Burkholderia cenocepacia to polymyxin B in isogenic strains with diverse polymyxin B resistance phenotypes

Gene (predicted function of encoded protein) Fold-Change
  Microarraya Real-Time PCRb
Genes differentially regulated in RSF34 compared to K56-2   
BCAL0114 (Flagellin) -5.8 No change
BCAL3490 (Exported protein) 12.0 6.8
BCAM0083 (Hypothetical protein) 12.1 10.0
BCAM0186 (Lectin) 14.4 9.8
BCAM0537 (Serine peptidase) 51.0 8.0
BCAM0855 (UDP-glucose dehydrogenase) 22.2 13.5
BCAM1010 (putative UTP-glucose-1-phosphate) 15.2 20.7
BCAM2453 (Redoxin) 40.5 6.2
Genes differentially regulated in RSF34 4000B compared to RSF34   
BCAL1083 (Exported alkaline phosphatase) -16.7 -3.2
BCAL1213 (2-oxoisovalerate dehydrogenase β subunit) 29.9 19.7
BCAL1270 (Phosphate transport, periplasmic) -23.5 -17.9
BCAM0537 (Serine peptidase) -28.2 -4.1
BCAM0855 (UDP-glucose dehydrogenase) -5.0 -4.2
BCAM2195 (AMP-binding enzyme) 22.8 4.9
BCAM2453 (Redoxin) -22.5 -1.9
Genes differentially regulated in K56-2 upon polymyxin B treatment   
BCAL0114 (Flagellin, FliC) -4.9 -10.3
BCAL0140 (Flagellar biosynthesis) -25.9 -26.2
BCAL0520 (Flagellar hook-length control, FlhB) -15.1 -131.9
BCAL0566 (Flagellar basal body rod modification, FlgD) -17.1 -7.7
BCAL1351 (Exported Protein) 3.6 31.3
BCAL3507 (FliL) -13.6 -4.3
BCAM0083 (Hypothetical protein) 21.4 34.5
BCAM2187 (Macrolide-specific ABC-type efflux) 9.5 33.7
  1. aData shown are the mean from three independent experiments.
  2. bData shown are the mean of two or three independent experiments, which are also independent of those used for microarray analysis.