Single-nucleotide resolution analysis of the transcriptome structure of Clostridium beijerinckii NCIMB 8052 using RNA-Seq

Table 1 Summary of RNA-Seq sequencing and data analysis results

Sample	1	2	3	4	5	6*	Total
Time collected (h)	2	4.5	10	14	17	26.5
Total No. of reads	8988633	9457480	8011531	8448929	10363535	38574501	83844609
No. of reads mapped	8473125	9037616	7514804	7730815	9842491	37676913	80275764
No. of reads unambiguously mapped	6776544	7274568	6096405	6189652	8096169	35027722	69461060
No. of bases unambiguously mapped	508240800	545592600	457230375	464223900	607212675	2627079150	5209579500
Percentage of genome represented	38.71	36.59	43.84	40.60	41.34	50.67	75.46
No. of genes with detectable expression**	4219	4082	4496	4453	4487	4750	5024
Range in expression levels (RPKM)	3.2 × 10^-1 ~ 2.5 × 10⁴	5.8 × 10^-2 ~ 6.0 × 10⁴	4.5 × 10^-2 ~ 2.5 × 10⁴	7.0 × 10^-2 ~ 9.0 × 10⁴	1.0 × 10^-1 ~ 8.6 × 10⁴	3.6 × 10^-2 ~ 9.8 × 10⁴	3.6 × 10^-2 ~ 9.8 × 10⁴

*Sample 6 was sequenced with 1 sample/lane, while samples 1-5 with 2 samples/lane. See Methods section for more details; **Pseudogenes included.

ISSN: 1471-2164