Transcriptome profiling of Pinus radiata juvenile wood with contrasting stiffness identifies putative candidate genes involved in microfibril orientation and cell wall mechanics

Table 2 Comparisons of gene ontology (GO) terms of unigenes differentially transcribed in juvenile wood with contrasting stiffness and microfibril angle

GO No.	GO Terms	Class ^a	HS ^b	LS ^b	P-value
GO:0030312	External encapsulating structure	CC	0	15	0.028
GO:0016469	Proton-transporting two-sector ATPase complex	CC	9	0	0.037
GO:0005774	Vacuolar membrane	CC	16	2	0.034
GO:0003779	Actin binding	MF	9	0	0.037
GO:0022804	Active transmembrane transporter activity	MF	32	11	0.041
GO:0016835	Carbon-oxygen lyase activity	MF	0	13	0.042
GO:0050662	Coenzyme binding	MF	0	20	0.013
GO:0005507	Copper ion binding	MF	9	0	0.037
GO:0020037	Heme binding	MF	16	2	0.034
GO:0004497	Monooxygenase activity	MF	9	0	0.037
GO:0004673	Protein histidine kinase activity	MF	9	0	0.037
GO:0046906	Tetrapyrrole binding	MF	16	2	0.034
GO:0046914	Transition metal ion binding	MF	68	35	0.048
GO:0003746	Translation elongation factor activity	MF	13	0	0.016
GO:0000155	Two-component sensor activity	MF	9	0	0.037
GO:0019752	Carboxylic acid metabolic process	BP	19	47	0.050
GO:0006812	Cation transport	BP	29	8	0.037
GO:0042180	Cellular ketone metabolic process	BP	19	47	0.050
GO:0044255	Cellular lipid metabolic process	BP	3	33	0.005
GO:0032502	Developmental process	BP	13	42	0.023
GO:0022900	Electron transport chain	BP	16	2	0.034
GO:0006631	Fatty acid metabolic process	BP	0	17	0.019
GO:0006811	Ion transport	BP	29	8	0.037
GO:0032787	Monocarboxylic acid metabolic process	BP	0	26	0.004
GO:0034470	ncRNA processing	BP	0	15	0.028
GO:0006082	Organic acid metabolic process	BP	19	47	0.050
GO:0009651	Response to salt stress	BP	19	4	0.049
GO:0050896	Response to stimulus	BP	91	51	0.040
GO:0006950	Response to stress	BP	68	33	0.032
GO:0009260	Ribonucleotide biosynthetic process	BP	19	4	0.049
GO:0006396	RNA processing	BP	3	26	0.015
GO:0006414	Translational elongation	BP	13	0	0.016

^aCC: cellular component; MF: molecular function; BP: biological process. ^bHS: wood with high stiffness and low microfibril angle; LS: wood with low stiffness and high microfibril angle; The number of GO terms assigned in the identified unigenes was normalized at 1, 000 unigenes.

ISSN: 1471-2164