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Table 1 Comparison of the ratios of transcript levels between WT and GPC-RNAi in the Illumina (12 DAA) and qRT-PCR experiments (12 DAA and 22 DAA).

From: Effect of the down-regulation of the high Grain Protein Content (GPC) genes on the wheat transcriptome during monocarpic senescence

  

qRT-PCR a

  

12 DAA

22 DAA

Isogroup

Illumina ratio

Ratio

P

ratio

P

Isogroup10136

8.745

4.976

0.004

6.472

0

Isogroup08662

5.887

2.11

0.018

2.021

0.001

Isogroup14133

3.303

0.983

0.955

1.208

0.78

Isogroup10811

3.226

2.183

0.034

4.795

0.005

Isogroup12718

2.917

1.67

0.189

1.8

0.018

Isogroup06043

2.797

1.761

0.04

2.088

0.045

Isogroup02905 b

2.292

1.349

0.192

2.8

0.008

Isogroup03083 b

2.218

0.839

0.638

1.904

0.108

Isogroup10053 b

2.19

1.017

0.92

1.094

0.572

Isogroup01211 b

2.052

0.806

0.506

1.582

0.119

Isogroup11278 b

0.471

0.764

0.18

0.726

0.114

Isogroup05843 b

0.448

0.852

0.316

0.827

0.068

Isogroup03470 b

0.445

0.863

0.271

0.845

0.063

Isogroup13287 b

0.422

1.012

0.942

0.853

0.427

Isogroup13088

0.393

0.698

0.050

0.843

0.492

Isogroup06482

0.365

0.916

0.461

0.924

0.605

Isogroup10940

0.333

0.739

0.018

0.631

0.917

Isogroup10620

0.272

0.716

0.029

1.068

0.732

Isogroup13722

0.267

0.606

0.005

0.522

0.336

Isogroup07898

0.254

0.97

0.816

0.882

0.325

Isogroup06574

0.237

0.973

0.782

0.759

0.063

Isogroup07736

0.199

0.62

0.015

0.975

0.912

  1. a Ratios presented as expression in WT/expression GPC-RNAi determined by Illumina counts and qRT-PCR. Ratios higher than one indicate genes up-regulated by senescence and ratios lower than one genes down-regulated by senescence. b Isogroups with less than 2.5 fold changes.
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BMC Genomics

ISSN: 1471-2164

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