Figure 1

Experimental design and replication. The experiments were conducted three times (top three red-outlined boxes). For each experiment, three hydroponic buckets were treated with control (NaOH and H₂O) or treatment (ABA and PEG) (middle grey box; colored diamond). After 27 hrs of treatment, 10 plants from each bucket were harvested, separated into roots and shoots, and RNA extracted (middle grey box; brown and green arrows). RNA samples from each bucket were combined in equimolar amounts and 5 μg of combined RNA used to create the Illumina RNA-Seq cDNA (bottom box; colored bins). Each flowcell (8 lanes) contained 4 root and 4 shoot samples, each having been treated with ABA, NaOH, PEG, or H₂O for 27 hrs. The order of the samples on the flowcell was assigned by random draw for each Illumina run.