A comparison of the 454 RNAseq (strain 44_PLY01) and EST (strain CCMP1788) sequence datasets. Sequences were assembled using CAP3 (see methods). Bottom panel: the contribution (as a proportion of the total contigs/ESTS per gene cluster) of 44_PLY01 contigs (red) and CCMP1788 ESTs (blue) to each gene cluster. Note - columns that are entirely red represent gene clusters composed of 44_PLY01 contigs only; entirely blue columns represent gene clusters composed of CCMP1788 ESTs only. Middle and top panels: the size (i.e. the number of contributing contigs and/or ESTs) distribution of gene variant clusters in the 44_PLY01 (middle panel) and CCMP1788 (top panel) datasets.