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Table 3 Primers used for each gene expression analysis by real-time PCR.

From: Effects of the total replacement of fish-based diet with plant-based diet on the hepatic transcriptome of two European sea bass (Dicentrarchus labrax) half-sibfamilies showing different growth rates with the plant-based diet

 

Forward primers (5'-3')

Reverse primers (5'-3')

amplicon size

FADS2

CCTTCACTGCTCTTCATCCCAA

CCCAGGTGGAGGCAGAAGAA

202

FABP7

GAAGGCACTTGGTGTTGGTT

CAGGGTTTTCACCACCACTT

102

HMGCR

CCAGCTTCGTATTCAGCACA

GCTTTGGAGAGGTCGATGAG

105

LPL

AGTTCCACATCCGGAAACTG

GCTCCGGTTGTCTTCTTTTG

142

GCK

GGTGAAGCAAGCCTGAACTC

CTTCCAGCAGTGACTGTCCA

122

ANGPTL3

CAACATCTTGCAGGAGCGTA

CTCTCCGACAGTCCCTTCAG

77

CXCL10

GGAGAGTGAGCCAGAACCTG

CCCTTGTGCACTGAAGACAA

91

EF1

GCTTCGAGGAAATCACCAAG

CAACCTTCCATCCCTTGAAC

153

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BMC Genomics

ISSN: 1471-2164

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