Figure 1

Circular representation of the C. jejuni NCTC11168 genome showing positions of transposon inserts. Outer 2 circles: open reading frames (purple) and pseudogenes (black) on the forward and reverse DNA strands. The next two circles show insert positions based on the Mariner library (dark red) and the Tn5 library (light red). The innermost circle shows %GC. Numbers on the outermost circle show the base number with respect to the start of dnaA (cj0001). Box outs show examples of the transposon mutagenesis analysis (genes are not drawn to scale). Genes are predicted essential if they lack any transposon insert (e.g. alaS or aroA) while genes with an insert are predicted to be dispensable for growth under laboratory conditions. The upper box out shows a region of the genome densely populated by transposon inserts and therefore this whole region is likely dispensable for growth under laboratory conditions. This region of the chromosome encodes functions relating to cell envelope biosynthesis and flagella modification.