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Table 4 Assessment of assembly reliability on randomly selected contigs by using PCR with primers designed from BAC end sequences

From: Generation of the first BAC-based physical map of the common carp genome

Contig ID Number of primer pairs Number of Clones Number of Positive Clones Contig assembly completely validated
2042 15 65 65 Yes
113 12 85 85 Yes
2092 8 34 34 Yes
348 2 11 11 Yes
4368 4 11 11 Yes
1494 4 25 25 Yes
2220 4 14 14 Yes
4074 2 10 10 Yes
1258 2 13 13 Yes
6064 2 13 13 Yes
3806 4 17 17 Yes
4498 4 13 13 Yes
677 6 37 37 Yes
2995 4 24 24 Yes
3682 4 22 22 Yes
5929 2 7 7 Yes
3097 2 12 12 Yes
984 2 12 12 Yes