Figure 3

Vailidation of pri-miRNA expression. Quantitative TaqMan^® RT-PCR of pri-miRNA expression in LNCaP and PrEC cells +/- 5-Aza-CdR treatment (n = 6, +/-SD, *p <0.05). Pri-miRNA RT-PCR assays followed established TaqMan^® workflows (Applied Biosystems). Briefly, total RNA was used for first-strand cDNA synthesis using 1 μg RNA Superscript reverse transcriptase (Invitrogen). cDNA was then subjected to 40 cycles of amplification using an ABI7900 instrument (Applied Biosystems). Data was normalized to 18S levels (2^-dCt).