Figure 1

Successful prediction of new CRMs. Reporter gene expression is shown in the left-hand panels (anti-GFP: A, C, E, G; GFP fluorescence, I), in situ hybridization to mRNA of the assigned target gene in the right-hand panels (B, D, F, H). Embryos are oriented anterior to the left and ventral side up (A-D) or laterally with dorsal to the top (G-H). (A) cooc164 drives reporter gene expression primarily in the ventral nerve cord in a pattern similar to that of target gene CG32105 (B). (C) cooc404 drives gene expression in the midline of the ventral nerve cord. (D) CG34347, putative target gene for cooc404, is expressed in the same midline cells (arrowheads). (E) Reporter gene expression from cooc102 can be observed throughout the mesoderm (black arrows, arrowheads). Expression in the visceral mesoderm (not shown) and anterior segments (white arrows) is not observed for the assigned target gene jhamt (F). Arrows and arrowheads in panel F mark somatic mesodermal cells corresponding to those similarly marked in panel E. (G) cooc310 reporter gene expression is observed in segmentally repeated stripes, primarily in the embryonic ectoderm. Inset shows cells in the mesoderm co-labeled (white cells, marked with arrows) for GFP (green) and the mesodermal marker Mef2 (magenta). (H) Corresponding stripes of expression are seen for target gene notum. (I) cooc110 drives gene expression in pupal tissues including the wing.