Phylogenetic relationship among the three clade representatives. A Neighbor Joining tree shows 100% bootstrap for clade A and B and for clade A and B. The bootstrap and NJ tree were calculated using MEGA 4.0 default parameters and the coding region of the maxicircle from 12S to partial ND5 for each strain. T. brucei and L. tarentolae were used as outgroups. Sylvio X10 is a representative of clade A; CL Brener is a representative of clade B; Esmeraldo is a representative of clade C.