The Trypanosoma cruzi Sylvio X10 strain maxicircle sequence: the third musketeer

BMC Genomics

Table 5 Primers for PCR amplification

Primer name	Primer sequence (5'-3')	Binding site	T_m	MgCl₂	Region amplified	Product
			(°C)	(mM)		(nt)
Tc. Sylvio.cons.Region.For1	TTGTAAAAACCTTATCAGCAAAGAAA	Palindrome	46	3.5	Palindrome-partial 12S	~2,000
Tc. Maxi.12S.rRNA.Rev	TTACTTGGTACATATATAACAACTG	12S
Tc.CLBmaxi12s.For2	GCACAGTTGTTATATATGTAC	12S	50	2.5	partial 12S- partial 9S	1,528
Tc.Sylvio.Maxi9s.2R	GATTACTGCACGTTATTTTTATT	9S
Tc.9s.For.unedited	GCCCACCAATTTTTATAATAA	9S	38	2.5	partial 9S-partial ND7	1,576
Tc.Maxi.ND8-Murf5.UTR.unedited.Rev	ATCCTTCGAACATCCCTCCT	ND7
Tc.ND7.For.unedited	CGGGAAGGAAGAACAGTT	ND7	37	2.5	partial ND7-partial Cyb	2,161
Tc.Cyb.Rev.CDS.not edited	CTAATCTAATCTACATACAAC	Cyb
Sylvio.Cyb.For.CDS.not edited	GTTGTATGTAGATTAGATTAG	Cyb	55	2.5	partial Cyb-partial ND1	2,773
Tc.CL Brener.NDI.CDS.Rev1	TTAATCTTATCAGGATTTGTTAGCC	ND1
Tc.CL Brener.NDI.CDS.For1	GGCTAACAAATCCTGATAAGATTAA	ND1	50	3.5	partial ND1-partial COI	3,419
Tc.Maxi.4.Rev2	TTGCTTAAGTGTTTCCCACAAA	COI
Tc.Maxi.4.For	TTTGTGGGAAACACTTAAGCAA	COI	40	2.5	partial COI-partial ND5	2,283
Tc.Maxi.ND3-RPS12.Rev.2	TTGATTGTCAAAAACTTATAAAATGCC	ND5
Tc. maxiRPS12-ND5.unedited.For1	CCAACTTCCCTTCAAACCAA	RPS12	50	2.5	partial RPS12-partial ND5	1,993
Tc.maxi.ND5.unedited.Rev1	TTCAAAATAACATAACAACATCCGTA	ND5

ISSN: 1471-2164