Table 6 Internal primers
From: The Trypanosoma cruzi Sylvio X10 strain maxicircle sequence: the third musketeer
Primer name | Primer sequence (5'-3') | Binding site |
|---|---|---|
99904 2-cons-12s F | CACAAGTTGTTATGCATGTAA | conserved region |
99905 2-cons-12s R | CTATCACAATTTGTGGGAAAA | conserved region |
83251 13-12S-9S F | CGAAAATAAAATTTTAGTAGCATA | 9S |
6713 B-9S-ND7 4F | GGTTGCCCTCTTGTTGTCAT | ND9 |
1-42 CO3 CYB (R) | CCTAAACTGAACCCCACTCC | Cyb |
1-43 CO3 CYB (R) | CCTAAACTGAACCCCACTCC | Cyb |
1-69 CO3CyB (R ) | CAAAAGCAAAGTCGCTCAC | Cyb |
1-68-CO3CyB (F) | TTGGAGTTGGGTGGAGTTC | ND7 |
55103 A Murf4-Cr3 R | CGATTATCTCAGAAAGTGCCTTA | ND1 |
64986 B-MURF4-CR3 3F | GGCAATGGGAATTGTACCTA | MURF1 |
6611 B-MRF4-CR3 2F | CGGGCAACAACGGTTTTGA | MURF4 (ATPase6) |
69245 COII-CO1 Maxi Sylvio F1 | ACCAATTTTGTATAACGCAATTATTA | MURF2 |
894-17F 4-COII-COI F | CGTATGCTTCTTAATATTATATTT | MURF2 |
894-18 4-COII-COI R | GCTTGATATAATGCTGCATGA | COI |
753-5 CO2-CO1 2F | CGGTATCAATTTTTTGATATA | MURF2 |
54597 CO2 CO1 2R | CGAGCATTATAAATTCTATTAA | COI |
55799 CO2-CO1 F | GTAGAGAACCGGGGAGGTGT | COII |
57799 CO2-CO1 R | TTGCACCTGTTATGGTTGGAT | COI |
1-58-ND4CR4Bfor-M13F_B08 | GGAGACTTTTTTACCAAGGG | ND3 |
1-59-ND4CR4Brev-M13R_CO8 | TCCCCCTTCTTCTCCTTCAC | CR4 |
259-67 RPS12-ND5 F | TGGCTAACCTTTTCATGTTCA | ND5 |
256-66 RPS12-ND5 R | CCTTGCAATAAAATCCACACAA | ND5 |